study on heavy metal resistant fecal coliforms isolated from industrial, urban uastewater in arak, iran

نویسندگان

a. akhavan sepahy

s. sharifian

m. r. zolfaghari

m. khalily dermany

h. rashedi

چکیده

in this essay, the heavy metal resistance patterns of bacterial strains isolated from industrial wastewater, domestic wastewater and various parts of the wastewater treatment system of arak city have been studied. 28 intestinal bacterial strains were screened and identified as klebsiella, escherichia coli, citrobacter and enterobacter species biochemical methods. the minimum inhibitory concentration (mic) against the various heavy metals including cadmium chloride, nickel sulfate, mercuric chloride, potassium chromate and potassium tellurite were determined using the agar dilution method and some antibiotics by disk method. in this study, for first time, we have found high mic for klebsiella sp., 22 mm (4032 μg/ml) for cadmium, 20 mm (3884 μg/ml) for chromium, 20 mm (6157 μg/ml) for telluride, 10 mm (2628 μg/ml) for nickel and 1mm (271.5 μg/ml) for mercury. furthermore, the bacterial strain escherichia coli dh5α was transformed with plasmid isolated from cd2+ resistant klebsiella sp. the same size of the plasmid was isolated from transformed e.coli dh5α and separated on 0.8% agarose gel electrophoresis. the curing was carried out by exposing cell culture overnight to sodium dodechyl sulphate, which resulted in losing their resistance to cadmium, and therefore, the ability of klebsiella sp. resistant to cadmium is plasmid mediated

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and Identification of Heavy Metal Resistant Bacteria from Industrial Wastewaters in Guilan Province

Heavy metal pollution by natural factors is a world-wide phenomenon. Release of large quantities of heavy metals without handling proper processes that could decrease the concentration of such a material is a hassle that makes strains resistant to these heavy metals apart from entering into human food chain. In this research, wastewater of four firms in Guilan province such as Foolad, Risandegi...

متن کامل

Isolation and Identification of Heavy Metal Resistant Bacteria from Industrial Wastewaters in Guilan Province

Heavy metal pollution by natural factors is a world-wide phenomenon. Release of large quantities of heavy metals without handling proper processes that could decrease the concentration of such a material is a hassle that makes strains resistant to these heavy metals apart from entering into human food chain. In this research, wastewater of four firms in Guilan province such as Foolad, Risandegi...

متن کامل

assessment of heavy metals contamination in urban topsoil from arak industrial city, iran

an increase in heavy metal pollution in the soils of arak due to industrialization and urbanization has become an environmental problem. the region hosts several industrial facilities which are the main source for hazardous wastes which include electric, metal, automotive supply industry, food, machinery, and chemicals. soil samples were collected from these industrial areas in the central and ...

متن کامل

Spatial distribution and degree of heavy metal pollution in urban roadside dusts from Sanandaj, Iran

Industrial activities and high traffic density are the most important heavy metal pollution sources in urban areas. Roadside dust created by atmospheric deposition can be one of the best indicators for heavy metal contamination levels. The present study reports the spatial distribution patterns and degree of heavy metal pollution (Cd, Cr, Ni, Pb, Zn, and As) in 50 roadside dust samples from urb...

متن کامل

Detection of Plasmids in Heavy Metal Resistance Bacteria Isolated from the Persian Gulf and Enclosed Industrial Areas

Several heavy metal resistant bacterial strains were isolated from sediment and water samples collected from the Persian Gulf and enclosed industrial areas. All the isolated bacteria were identified by 16S rRNA gene sequencing. Isolated bacteria were tested for the presence of plasmids using the modified alkaline lysate method. The method was effective for identification and characterization of...

متن کامل

Clonal Diversity in Multi Drug Resistant (MDR) Enterococci Isolated from Fecal Normal Flora

Enterococci are Gram positive and catalase- negative cocci that are found in the gastrointestinal tract of mammals and birds, and are readily isolated from soil, surface and waters. The aim of this study was to discriminate between Enterococcus isolates based on repetitive element sequence based –PCR (Rep-PCR) with the BOXA2R primer and their antibiotics profile. Enterococci isolates were obtai...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of environmental research

ناشر: university of tehran

ISSN 1735-6865

دوره 9

شماره 4 2015

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023